e coli ydfg insert (New England Biolabs)
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e coli ydfg insert
E Coli Ydfg Insert, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6939 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e+coli+ydfg+insert/XbaI/us12054721-1498-62-78
Average 99 stars, based on 6939 article reviews
E Coli Ydfg Insert, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6939 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e+coli+ydfg+insert/XbaI/us12054721-1498-62-78
Average 99 stars, based on 6939 article reviews
e coli ydfg insert - by Bioz Stars,
2026-11
99/100 stars
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Expressing:Article Title: Compositions and methods for 3-hydroxypropionic acid production Article Snippet: Right-Hand Fragment Containing E. coli 3-HPDH (SEQ ID NO: 143) 2 μg of a mini-prep of pMhCt069 (supra) was digested with XbaI and PacI, treated with 10 units calf intestinal phosphatase (New England Biolabs) at 37° C. for 30 minutes, and purified by 0.9% agarose gel electrophoresis in TAE buffer, and an approximately 2.2 kbp band was excised from the gel and purified using a NUCLEOSPIN® Extract II Kit (Macherey-Nagel) according to the manufacturer's instructions. .. A fragment digested with XbaI and PacI containing E. coli 3-HPDH gene codon-optimized for expression in I. orientalis (ydfG, SEQ ID NO: 143) was ligated into the pMhCt069 cut vector as follows: A ligation reaction (20 μL) containing 1× Quick ligation buffer (New England Biolabs), 2 μL of the purified pMhCt069 vector digested with XbaI and PacI, 4 μL of the Article Title: Compositions and methods for 3-hydroxypropionic acid production Article Snippet: Right-Hand Fragment Containing E. coli 3-HPDH (SEQ ID NO: 143) 2 μg of a mini-prep of pMhCt069 (supra) was digested with XbaI and Pad, treated with 10 units calf intestinal phosphatase (New England Biolabs) at 37° C. for 30 minutes, and purified by 0.9% agarose gel electrophoresis in TAE buffer, and an approximately 2.2 kbp band was excised from the gel and purified using a NUCLEOSPIN® Extract 11 Kit (Macherey-Nagel) according to the manufacturer's instructions. .. A fragment digested with XbaI and Pad containing E. coli 3-HPDH gene codon-optimized for expression in I. orientalis (ydfG, SEQ ID NO: 143) was ligated into the pMhCt069 cut vector as follows: A ligation reaction (20 μL) containing 1× Quick ligation buffer (New England Biolabs), 2 μL of the purified pMhCt069 vector digested with XbaI and Pad, 4 μL of the Article Title: Compositions and methods for 3-hydroxypropionic acid production Article Snippet: Right-Hand Fragment Containing E. coli 3-HPDH (SEQ ID NO: 143) 2 μg of a mini-prep of pMhCt069 (supra) was digested with XbaI and PacI, treated with 10 units calf intestinal phosphatase (New England Biolabs) at 37° C. for 30 minutes, and purified by 0.9% agarose gel electrophoresis in TAE buffer, and an approximately 2.2 kbp band was excised from the gel and purified using a NUCLEOSPIN® Extract II Kit (Macherey-Nagel) according to the manufacturer's instructions. .. A fragment digested with XbaI and PacI containing E. coli 3-HPDH gene codon-optimized for expression in I. orientalis (ydfG, SEQ ID NO: 143) was ligated into the pMhCt069 cut vector as follows: A ligation reaction (20 μL) containing 1× Quick ligation buffer (New England Biolabs), 2 μL of the purified pMhCt069 vector digested with XbaI and PacI, 4 μL of the Article Title: Compositions and methods for 3-hydroxypropionic acid production Article Snippet: Right-Hand Fragment Containing E. coli 3-HPDH (SEQ ID NO: 143) 2 μg of a mini-prep of pMhCt069 (supra) was digested with XbaI and PacI, treated with 10 units calf intestinal phosphatase (New England Biolabs) at 37° C. for 30 minutes, and purified by 0.9% agarose gel electrophoresis in TAE buffer, and an approximately 2.2 kbp band was excised from the gel and purified using a NUCLEOSPIN® Extract II Kit (Macherey-Nagel) according to the manufacturer's instructions. .. A fragment digested with XbaI and PacI containing E. coli 3-HPDH gene codon-optimized for expression in I. orientalis (ydfG, SEQ ID NO: 143) was ligated into the pMhCt069 cut vector as follows: A ligation reaction (20 μL) containing 1× Quick ligation buffer (New England Biolabs), 2 μL of the purified pMhCt069 vector digested with XbaI and PacI, 4 μL of the Article Title: Compositions and methods for 3-hydroxypropionic acid production Article Snippet: Right-Hand Fragment Containing E. coli 3-HPDH (SEQ ID NO: 143) 2 μg of a mini-prep of pMhCt069 (supra) was digested with XbaI and PacI, treated with 10 units calf intestinal phosphatase (New England Biolabs) at 37° C. for 30 minutes, and purified by 0.9% agarose gel electrophoresis in TAE buffer, and an approximately 2.2 kbp band was excised from the gel and purified using a NUCLEOSPIN® Extract II Kit (Macherey-Nagel) according to the manufacturer's instructions. .. A fragment digested with XbaI and PacI containing E. coli 3-HPDH gene codon-optimized for expression in I. orientalis (ydfG, SEQ ID NO: 143) was ligated into the pMhCt069 cut vector as follows: A ligation reaction (20 μL) containing 1× Quick ligation buffer (New England Biolabs), 2 μL of the purified pMhCt069 vector digested with XbaI and PacI, 4 μL of the Article Title: Compositions and methods for 3-hydroxypropionic acid production Article Snippet: 2 μg of a mini-prep of pMhCt069 (supra) was digested with XbaI and PacI, treated with 10 units calf intestinal phosphatase (New England Biolabs) at 37° C. for 30 minutes, and purified by 0.9% agarose gel electrophoresis in TAE buffer, and an approximately 2.2 kbp band was excised from the gel and purified using a NUCLEOSPIN® Extract II Kit (Macherey-Nagel) according to the manufacturer's instructions. .. A fragment digested with XbaI and PacI containing E. coli 3-HPDH gene codon-optimized for expression in I. orientalis (ydfG, SEQ ID NO: 143) was ligated into the pMhCt069 cut vector as follows: A ligation reaction (20 μL) containing 1× Quick ligation buffer (New England Biolabs), 2 μL of the purified pMhCt069 vector digested with XbaI and PacI, 4 μL of the Ligation:Article Title: Compositions and methods for 3-hydroxypropionic acid production Article Snippet: Right-Hand Fragment Containing E. coli 3-HPDH (SEQ ID NO: 143) 2 μg of a mini-prep of pMhCt069 (supra) was digested with XbaI and PacI, treated with 10 units calf intestinal phosphatase (New England Biolabs) at 37° C. for 30 minutes, and purified by 0.9% agarose gel electrophoresis in TAE buffer, and an approximately 2.2 kbp band was excised from the gel and purified using a NUCLEOSPIN® Extract II Kit (Macherey-Nagel) according to the manufacturer's instructions. .. A fragment digested with XbaI and PacI containing E. coli 3-HPDH gene codon-optimized for expression in I. orientalis (ydfG, SEQ ID NO: 143) was ligated into the pMhCt069 cut vector as follows: A ligation reaction (20 μL) containing 1× Quick ligation buffer (New England Biolabs), 2 μL of the purified pMhCt069 vector digested with XbaI and PacI, 4 μL of the Article Title: Compositions and methods for 3-hydroxypropionic acid production Article Snippet: Right-Hand Fragment Containing E. coli 3-HPDH (SEQ ID NO: 143) 2 μg of a mini-prep of pMhCt069 (supra) was digested with XbaI and Pad, treated with 10 units calf intestinal phosphatase (New England Biolabs) at 37° C. for 30 minutes, and purified by 0.9% agarose gel electrophoresis in TAE buffer, and an approximately 2.2 kbp band was excised from the gel and purified using a NUCLEOSPIN® Extract 11 Kit (Macherey-Nagel) according to the manufacturer's instructions. .. A fragment digested with XbaI and Pad containing E. coli 3-HPDH gene codon-optimized for expression in I. orientalis (ydfG, SEQ ID NO: 143) was ligated into the pMhCt069 cut vector as follows: A ligation reaction (20 μL) containing 1× Quick ligation buffer (New England Biolabs), 2 μL of the purified pMhCt069 vector digested with XbaI and Pad, 4 μL of the Article Title: Compositions and methods for 3-hydroxypropionic acid production Article Snippet: Right-Hand Fragment Containing E. coli 3-HPDH (SEQ ID NO: 143) 2 μg of a mini-prep of pMhCt069 (supra) was digested with XbaI and PacI, treated with 10 units calf intestinal phosphatase (New England Biolabs) at 37° C. for 30 minutes, and purified by 0.9% agarose gel electrophoresis in TAE buffer, and an approximately 2.2 kbp band was excised from the gel and purified using a NUCLEOSPIN® Extract II Kit (Macherey-Nagel) according to the manufacturer's instructions. .. A fragment digested with XbaI and PacI containing E. coli 3-HPDH gene codon-optimized for expression in I. orientalis (ydfG, SEQ ID NO: 143) was ligated into the pMhCt069 cut vector as follows: A ligation reaction (20 μL) containing 1× Quick ligation buffer (New England Biolabs), 2 μL of the purified pMhCt069 vector digested with XbaI and PacI, 4 μL of the Article Title: Compositions and methods for 3-hydroxypropionic acid production Article Snippet: Right-Hand Fragment Containing E. coli 3-HPDH (SEQ ID NO: 143) 2 μg of a mini-prep of pMhCt069 (supra) was digested with XbaI and PacI, treated with 10 units calf intestinal phosphatase (New England Biolabs) at 37° C. for 30 minutes, and purified by 0.9% agarose gel electrophoresis in TAE buffer, and an approximately 2.2 kbp band was excised from the gel and purified using a NUCLEOSPIN® Extract II Kit (Macherey-Nagel) according to the manufacturer's instructions. .. A fragment digested with XbaI and PacI containing E. coli 3-HPDH gene codon-optimized for expression in I. orientalis (ydfG, SEQ ID NO: 143) was ligated into the pMhCt069 cut vector as follows: A ligation reaction (20 μL) containing 1× Quick ligation buffer (New England Biolabs), 2 μL of the purified pMhCt069 vector digested with XbaI and PacI, 4 μL of the Article Title: Compositions and methods for 3-hydroxypropionic acid production Article Snippet: Right-Hand Fragment Containing E. coli 3-HPDH (SEQ ID NO: 143) 2 μg of a mini-prep of pMhCt069 (supra) was digested with XbaI and PacI, treated with 10 units calf intestinal phosphatase (New England Biolabs) at 37° C. for 30 minutes, and purified by 0.9% agarose gel electrophoresis in TAE buffer, and an approximately 2.2 kbp band was excised from the gel and purified using a NUCLEOSPIN® Extract II Kit (Macherey-Nagel) according to the manufacturer's instructions. .. A fragment digested with XbaI and PacI containing E. coli 3-HPDH gene codon-optimized for expression in I. orientalis (ydfG, SEQ ID NO: 143) was ligated into the pMhCt069 cut vector as follows: A ligation reaction (20 μL) containing 1× Quick ligation buffer (New England Biolabs), 2 μL of the purified pMhCt069 vector digested with XbaI and PacI, 4 μL of the Article Title: Compositions and methods for 3-hydroxypropionic acid production Article Snippet: 2 μg of a mini-prep of pMhCt069 (supra) was digested with XbaI and PacI, treated with 10 units calf intestinal phosphatase (New England Biolabs) at 37° C. for 30 minutes, and purified by 0.9% agarose gel electrophoresis in TAE buffer, and an approximately 2.2 kbp band was excised from the gel and purified using a NUCLEOSPIN® Extract II Kit (Macherey-Nagel) according to the manufacturer's instructions. .. A fragment digested with XbaI and PacI containing E. coli 3-HPDH gene codon-optimized for expression in I. orientalis (ydfG, SEQ ID NO: 143) was ligated into the pMhCt069 cut vector as follows: A ligation reaction (20 μL) containing 1× Quick ligation buffer (New England Biolabs), 2 μL of the purified pMhCt069 vector digested with XbaI and PacI, 4 μL of the Purification:Article Title: Compositions and methods for 3-hydroxypropionic acid production Article Snippet: Right-Hand Fragment Containing E. coli 3-HPDH (SEQ ID NO: 143) 2 μg of a mini-prep of pMhCt069 (supra) was digested with XbaI and PacI, treated with 10 units calf intestinal phosphatase (New England Biolabs) at 37° C. for 30 minutes, and purified by 0.9% agarose gel electrophoresis in TAE buffer, and an approximately 2.2 kbp band was excised from the gel and purified using a NUCLEOSPIN® Extract II Kit (Macherey-Nagel) according to the manufacturer's instructions. .. A fragment digested with XbaI and PacI containing E. coli 3-HPDH gene codon-optimized for expression in I. orientalis (ydfG, SEQ ID NO: 143) was ligated into the pMhCt069 cut vector as follows: A ligation reaction (20 μL) containing 1× Quick ligation buffer (New England Biolabs), 2 μL of the purified pMhCt069 vector digested with XbaI and PacI, 4 μL of the Article Title: Compositions and methods for 3-hydroxypropionic acid production Article Snippet: Right-Hand Fragment Containing E. coli 3-HPDH (SEQ ID NO: 143) 2 μg of a mini-prep of pMhCt069 (supra) was digested with XbaI and Pad, treated with 10 units calf intestinal phosphatase (New England Biolabs) at 37° C. for 30 minutes, and purified by 0.9% agarose gel electrophoresis in TAE buffer, and an approximately 2.2 kbp band was excised from the gel and purified using a NUCLEOSPIN® Extract 11 Kit (Macherey-Nagel) according to the manufacturer's instructions. .. A fragment digested with XbaI and Pad containing E. coli 3-HPDH gene codon-optimized for expression in I. orientalis (ydfG, SEQ ID NO: 143) was ligated into the pMhCt069 cut vector as follows: A ligation reaction (20 μL) containing 1× Quick ligation buffer (New England Biolabs), 2 μL of the purified pMhCt069 vector digested with XbaI and Pad, 4 μL of the Article Title: Compositions and methods for 3-hydroxypropionic acid production Article Snippet: Right-Hand Fragment Containing E. coli 3-HPDH (SEQ ID NO: 143) 2 μg of a mini-prep of pMhCt069 (supra) was digested with XbaI and PacI, treated with 10 units calf intestinal phosphatase (New England Biolabs) at 37° C. for 30 minutes, and purified by 0.9% agarose gel electrophoresis in TAE buffer, and an approximately 2.2 kbp band was excised from the gel and purified using a NUCLEOSPIN® Extract II Kit (Macherey-Nagel) according to the manufacturer's instructions. .. A fragment digested with XbaI and PacI containing E. coli 3-HPDH gene codon-optimized for expression in I. orientalis (ydfG, SEQ ID NO: 143) was ligated into the pMhCt069 cut vector as follows: A ligation reaction (20 μL) containing 1× Quick ligation buffer (New England Biolabs), 2 μL of the purified pMhCt069 vector digested with XbaI and PacI, 4 μL of the Article Title: Compositions and methods for 3-hydroxypropionic acid production Article Snippet: Right-Hand Fragment Containing E. coli 3-HPDH (SEQ ID NO: 143) 2 μg of a mini-prep of pMhCt069 (supra) was digested with XbaI and PacI, treated with 10 units calf intestinal phosphatase (New England Biolabs) at 37° C. for 30 minutes, and purified by 0.9% agarose gel electrophoresis in TAE buffer, and an approximately 2.2 kbp band was excised from the gel and purified using a NUCLEOSPIN® Extract II Kit (Macherey-Nagel) according to the manufacturer's instructions. .. A fragment digested with XbaI and PacI containing E. coli 3-HPDH gene codon-optimized for expression in I. orientalis (ydfG, SEQ ID NO: 143) was ligated into the pMhCt069 cut vector as follows: A ligation reaction (20 μL) containing 1× Quick ligation buffer (New England Biolabs), 2 μL of the purified pMhCt069 vector digested with XbaI and PacI, 4 μL of the Article Title: Compositions and methods for 3-hydroxypropionic acid production Article Snippet: Right-Hand Fragment Containing E. coli 3-HPDH (SEQ ID NO: 143) 2 μg of a mini-prep of pMhCt069 (supra) was digested with XbaI and PacI, treated with 10 units calf intestinal phosphatase (New England Biolabs) at 37° C. for 30 minutes, and purified by 0.9% agarose gel electrophoresis in TAE buffer, and an approximately 2.2 kbp band was excised from the gel and purified using a NUCLEOSPIN® Extract II Kit (Macherey-Nagel) according to the manufacturer's instructions. .. A fragment digested with XbaI and PacI containing E. coli 3-HPDH gene codon-optimized for expression in I. orientalis (ydfG, SEQ ID NO: 143) was ligated into the pMhCt069 cut vector as follows: A ligation reaction (20 μL) containing 1× Quick ligation buffer (New England Biolabs), 2 μL of the purified pMhCt069 vector digested with XbaI and PacI, 4 μL of the Article Title: Compositions and methods for 3-hydroxypropionic acid production Article Snippet: 2 μg of a mini-prep of pMhCt069 (supra) was digested with XbaI and PacI, treated with 10 units calf intestinal phosphatase (New England Biolabs) at 37° C. for 30 minutes, and purified by 0.9% agarose gel electrophoresis in TAE buffer, and an approximately 2.2 kbp band was excised from the gel and purified using a NUCLEOSPIN® Extract II Kit (Macherey-Nagel) according to the manufacturer's instructions. .. A fragment digested with XbaI and PacI containing E. coli 3-HPDH gene codon-optimized for expression in I. orientalis (ydfG, SEQ ID NO: 143) was ligated into the pMhCt069 cut vector as follows: A ligation reaction (20 μL) containing 1× Quick ligation buffer (New England Biolabs), 2 μL of the purified pMhCt069 vector digested with XbaI and PacI, 4 μL of the |